3QW0
Crystal structure of the Zn-RIDC1 complex stabilized by BMB crosslinks
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-08 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.626, 76.399, 93.265 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.500 - 1.840 |
| R-factor | 0.1936 |
| Rwork | 0.190 |
| R-free | 0.24320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.955 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.550 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 39905 | |
| <I/σ(I)> | 3.19 | |
| Completeness [%] | 99.4 | 97.9 |
| Redundancy | 3.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 100 mM Bis-TRIS, 14% PEG 3350, 2.84 mM zinc chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
