3QVY

Crystal structure of the Zn-RIDC1 complex stabilized by BMOE crosslinks

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-10
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 61
Unit cell lengths	52.088, 52.088, 253.943
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.510 - 2.300
R-factor	0.2453
Rwork	0.242
R-free	0.28620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.025
RMSD bond angle	1.805
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	253.943	42.507	2.420
High resolution limit [Å]	2.300	7.270	2.300
Rmerge		0.031	0.248
Total number of observations		5690	27506
Number of reflections	17118
<I/σ(I)>	31.7	17.8	3.1
Completeness [%]	99.3	95.6	99.1
Redundancy	11.1	10.8	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	100 mM HEPES, 10% PEG 3350, 4.6 mM zinc chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K