3QVR
Crystal structure of glucose oxidase for space group P3121 at 1.3 A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 65.952, 65.952, 214.252 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.060 - 1.300 |
R-factor | 0.18 |
Rwork | 0.180 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cf3 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP (from ccp4) |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.460 | 1.320 |
High resolution limit [Å] | 1.300 | 1.430 | 1.300 |
Rmerge | 0.056 | 0.233 | 0.321 |
Number of reflections | 132764 | ||
<I/σ(I)> | 29.9 | 6.1 | 3.6 |
Completeness [%] | 99.2 | 99.3 | 98.7 |
Redundancy | 8 | 6.6 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 295 | 200 mM CaCl2 and 20% PEG 3350, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K |