3QVP

Crystal structure of glucose oxidase for space group C2221 at 1.2 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9
Spacegroup name	C 2 2 21
Unit cell lengths	69.560, 119.147, 142.188
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.610 - 1.200
R-factor	0.178
Rwork	0.178
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cf3
RMSD bond length	0.012
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP (from ccp4)
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	1.350	1.220
High resolution limit [Å]	1.200	1.320	1.200
Rmerge	0.066	0.221	0.289
Number of reflections	179408
<I/σ(I)>	23.1	6.2	4.1
Completeness [%]	97.0	98.4	97.8
Redundancy	5.8	4.9	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	295	200 mM NaCl and 20% PEG 3350, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K