3QVM
The structure of olei00960, a hydrolase from Oleispira antarctica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 4 |
| Unit cell lengths | 123.067, 123.067, 49.218 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.767 - 1.998 |
| R-factor | 0.1571 |
| Rwork | 0.156 |
| R-free | 0.18470 |
| Structure solution method | PHASER |
| Starting model (for MR) | Model based on 1wom |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.964 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.122 | 0.531 |
| Number of reflections | 50298 | |
| <I/σ(I)> | 24.75 | 4.13 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 7.7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.05M Calcium Chloride, 0.1M Bis-Tris pH6.5, 1.6M Ammonium Sulfate. Cryoprotected with Paratone-N oil, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
