3QVH
Crystal structure of fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.38079 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 87.670, 87.670, 78.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.210 - 1.850 |
| R-factor | 0.16568 |
| Rwork | 0.165 |
| R-free | 0.21329 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d40 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.044 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 30430 | |
| <I/σ(I)> | 12.3 | 1.6 |
| Completeness [%] | 98.6 | 93.2 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 17 % PEG3350, 25 % glycerol, 0.1 M MES, 10 mM ADP, 50 mM MgCl2 , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
