3QUW
Crystal structure of yeast Mmf1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-30 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97012 |
| Spacegroup name | H 3 |
| Unit cell lengths | 77.618, 77.618, 43.911 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.810 - 1.750 |
| R-factor | 0.18303 |
| Rwork | 0.181 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jd1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.139 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.104 | 0.250 |
| Number of reflections | 9969 | |
| <I/σ(I)> | 14.8 | 7.2 |
| Completeness [%] | 99.3 | 94.5 |
| Redundancy | 11 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.1 M Bis-Tris, 25% polyethylene glycol 3550, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
