3QUS
Crystal Structure of N10-Formyltetrahydrofolate Synthetase with ATPgS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 2 |
Unit cell lengths | 160.840, 160.840, 256.479 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 122.400 - 2.840 |
R-factor | 0.221 |
Rwork | 0.217 |
R-free | 0.28860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3PZX |
RMSD bond length | 0.014 |
RMSD bond angle | 1.657 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 122.405 | 2.890 |
High resolution limit [Å] | 2.840 | 2.840 |
Rmerge | 0.148 | 0.490 |
Number of reflections | 30394 | |
<I/σ(I)> | 6.1 | |
Completeness [%] | 97.0 | 99.8 |
Redundancy | 11.7 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | 50-75 mM Potassium Maleate Buffer pH 7.0-8.0, 1 mM dithiothreitol, 38-46% Ammonium Sulfate, 1-3.5% PEG 1000 or PEG 1450, VAPOR DIFFUSION, HANGING DROP, temperature 278K |