3QUN
Crystal structure of fosfomycin resistance kinase FomA from Streptomyces wedmorensis complexed with MgATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CAMD BEAMLINE GCPCC |
Synchrotron site | CAMD |
Beamline | GCPCC |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-16 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.38079 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 85.876, 85.876, 80.152 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.010 - 1.870 |
R-factor | 0.18527 |
Rwork | 0.185 |
R-free | 0.20541 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d40 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.763 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.940 |
High resolution limit [Å] | 1.870 | 1.870 |
Number of reflections | 26774 | |
<I/σ(I)> | 32 | 1.9 |
Completeness [%] | 93.4 | 96.9 |
Redundancy | 2.9 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 17% PEG3350, 15% glycerol, 0.1M MES, 10mM ATP, 10 mM MgCl2, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |