3QUJ
Crystal structure of the phosphonate binding protein, PhnD, from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2009-11-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.768, 64.399, 107.688 |
| Unit cell angles | 90.00, 93.52, 90.00 |
Refinement procedure
| Resolution | 28.900 - 2.200 |
| R-factor | 0.20586 |
| Rwork | 0.202 |
| R-free | 0.27640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.624 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (for MR) |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.900 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 55007 |
| Completeness [%] | 99.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2M Magnesium chloride hexahydrate, 0.1M Bis-tris, 25%(w/v) PEG 3,350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
