3QUI
Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.918410 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.083, 75.862, 93.437 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.577 - 1.933 |
| R-factor | 0.2168 |
| Rwork | 0.213 |
| R-free | 0.28720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1t |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.116 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.040 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.076 | 0.451 |
| Number of reflections | 29725 | |
| <I/σ(I)> | 9.4 | 1.97 |
| Completeness [%] | 89.8 | 66.2 |
| Redundancy | 3.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 200 mM ammonium sulphate, 200 mM NaCl, 50 mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
