3QUI
Crystal structure of Pseudomonas aeruginosa Hfq in complex with ADPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.918410 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.083, 75.862, 93.437 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.577 - 1.933 |
R-factor | 0.2168 |
Rwork | 0.213 |
R-free | 0.28720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u1t |
RMSD bond length | 0.007 |
RMSD bond angle | 1.116 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.040 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.076 | 0.451 |
Number of reflections | 29725 | |
<I/σ(I)> | 9.4 | 1.97 |
Completeness [%] | 89.8 | 66.2 |
Redundancy | 3.2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 200 mM ammonium sulphate, 200 mM NaCl, 50 mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |