3QTM
Structure of S. pombe nuclear import adaptor Nro1 (Space group P21)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791, 0.97946, 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.930, 107.840, 101.790 |
| Unit cell angles | 90.00, 102.29, 90.00 |
Refinement procedure
| Resolution | 39.900 - 2.150 |
| R-factor | 0.1797 |
| Rwork | 0.178 |
| R-free | 0.21310 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.300 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 55704 | |
| <I/σ(I)> | 6.3 | 1.7 |
| Completeness [%] | 97.4 | 86.8 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M Hepes pH 7.0, 1.6M ammonium sulphate, 10mM MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
