3QTH
Crystal structure of a DinB-like protein (CPS_3021) from Colwellia psychrerythraea 34H at 2.20 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-28 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.112, 75.026, 101.404 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.159 - 2.200 |
R-factor | 0.183 |
Rwork | 0.182 |
R-free | 0.20890 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | SOLVE |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.159 | 29.159 | 2.260 |
High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
Rmerge | 0.050 | 0.941 | |
Total number of observations | 1631 | 10860 | |
Number of reflections | 20863 | ||
<I/σ(I)> | 10.4 | 12.5 | 0.8 |
Completeness [%] | 100.0 | 95.8 | 100 |
Redundancy | 7.2 | 5.9 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.700M (NH4)2SO4, 15.00% Glycerol, 1.700% PEG-400, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |