3QTA
Crystal structure of a CheC-like protein (rrnAC0528) from Haloarcula marismortui ATCC 43049 at 2.00 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-27 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97958,0.97885 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 89.928, 89.928, 148.299 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.976 - 2.000 |
R-factor | 0.1762 |
Rwork | 0.175 |
R-free | 0.20230 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.488 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.976 | 29.976 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.011 | 0.011 | |
Total number of observations | 3346 | 22338 | |
Number of reflections | 41896 | ||
<I/σ(I)> | 8.9 | 25.8 | 2 |
Completeness [%] | 100.0 | 97.5 | 100 |
Redundancy | 7.3 | 6 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.16M Ca(OAc)2, 20.0% Glycerol, 14.4% PEG-8000, 0.1M Cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |