3QT9

Analysis of a new family of widely distributed metal-independent alpha mannosidases provides unique insight into the processing of N-linked glycans, Clostridium perfringens CPE0426 complexed with alpha-1,6-linked 1-thio-alpha-mannobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	117
Detector technology	CCD
Collection date	2010-01-01
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	49.870, 96.590, 109.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.050
R-factor	0.15999
Rwork	0.158
R-free	0.20405
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.391
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.150	2.160
High resolution limit [Å]	2.050	2.050
Number of reflections	28513
Completeness [%]	99.0	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5		0.1 M MES, 12% polyethylene glycol (PEG) 20,000, pH 6.5, VAPOR DIFFUSION, HANGING DROP