3QSZ
Crystal Structure of the STAR-related lipid transfer protein (fragment 25-204) from Xanthomonas axonopodis at the resolution 2.4A, Northeast Structural Genomics Consortium Target XaR342
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-16 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.115, 53.885, 70.266 |
| Unit cell angles | 90.00, 93.44, 90.00 |
Refinement procedure
| Resolution | 29.762 - 2.389 |
| R-factor | 0.2075 |
| Rwork | 0.201 |
| R-free | 0.26440 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.126 |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.389 | 2.400 |
| Rmerge | 0.082 | 0.461 |
| Number of reflections | 35747 | |
| <I/σ(I)> | 18.1 | 2.2 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | macrobatch under oik | 6 | 293 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution:0.1M MnSO4, 0.1M MES, 18% (w/v) PEG 4K, macrobatch under oik, temperature 293K |
