3QSY
Recognition of the methionylated initiator tRNA by the translation initiation factor 2 in Archaea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 198.3 |
Detector technology | CCD |
Collection date | 2008-10-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 93.680, 93.680, 220.030 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.950 - 3.200 |
R-factor | 0.2786 |
Rwork | 0.278 |
R-free | 0.30250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aho |
RMSD bond length | 0.009 |
RMSD bond angle | 1.307 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.400 | 3.100 |
High resolution limit [Å] | 3.000 | 3.300 | 3.000 |
Number of reflections | 23068 | ||
Completeness [%] | 99.6 | 100 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 45% ammonium sulphate, 30mM Tris-HCl, 5mM MES, 7mM MgCl2, 0.5mM DTT, 0.2mM GDPNP, 300mM NDSB 195, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285.0K |