3QSP
Analysis of a new family of widely distributed metal-independent alpha mannosidases provides unique insight into the processing of N-linked glycans, Streptococcus pneumoniae SP_2144 non-productive substrate complex with alpha-1,6-mannobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-002+ |
Temperature [K] | 117 |
Detector technology | IMAGE PLATE |
Collection date | 2010-01-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54180 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.510, 159.040, 60.130 |
Unit cell angles | 90.00, 106.74, 90.00 |
Refinement procedure
Resolution | 79.520 - 2.100 |
R-factor | 0.15971 |
Rwork | 0.156 |
R-free | 0.22021 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.415 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.520 | 2.155 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 52816 | |
Completeness [%] | 99.0 | 99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 0.1 M Hepes, 1.2 M LiSO4, pH 8.0, VAPOR DIFFUSION, HANGING DROP |