3QS4
Crystal structure of LeuT mutant F259V bound to sodium and L-tryptophan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97625 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.219, 86.595, 82.058 |
Unit cell angles | 90.00, 93.63, 90.00 |
Refinement procedure
Resolution | 45.019 - 2.631 |
R-factor | 0.2148 |
Rwork | 0.214 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f3a |
RMSD bond length | 0.002 |
RMSD bond angle | 0.559 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 2010_04_18_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.740 |
High resolution limit [Å] | 2.630 | 2.630 |
Number of reflections | 18602 | |
<I/σ(I)> | 16.1 | 2 |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES, 0.2 M NaCl, 24-26 % PEG-MME 550, 0.05 M L-tryptophan, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |