3QS2
Crystal structure of the biofilm forming subunit of the E. coli common pilus: full length domain swapped dimer of EcpA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-24 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.860, 72.080, 108.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.010 - 1.780 |
R-factor | 0.183 |
Rwork | 0.179 |
R-free | 0.22000 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.357 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.6.0104) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.010 | 1.880 |
High resolution limit [Å] | 1.780 | 1.780 |
Number of reflections | 35831 | |
<I/σ(I)> | 3.7 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 26.5 | 26.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 3.2 | 293 | 15% PEG 3350, 100 mM phosphate/citrate, 200 mM NaI, pH 3.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |