3QRR
Structure of Thermus Thermophilus Cse3 bound to an RNA representing a product complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-11 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.11588 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 62.170, 68.747, 155.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.111 - 3.099 |
R-factor | 0.2479 |
Rwork | 0.245 |
R-free | 0.30030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.657 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC (5.5.0110) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.111 | 3.179 |
High resolution limit [Å] | 3.099 | 3.099 |
Number of reflections | 6268 | |
Completeness [%] | 98.6 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | vapour diffusion, hanging drop | 7.5 | 298 | 15% PEG 3350, 100mM KOAc, 1mM spermidine, pH 7.5, vapour diffusion, hanging drop, temperature 298K |