3QRR
Structure of Thermus Thermophilus Cse3 bound to an RNA representing a product complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-11 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.11588 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.170, 68.747, 155.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.111 - 3.099 |
| R-factor | 0.2479 |
| Rwork | 0.245 |
| R-free | 0.30030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.657 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC (5.5.0110) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.111 | 3.179 |
| High resolution limit [Å] | 3.099 | 3.099 |
| Number of reflections | 6268 | |
| Completeness [%] | 98.6 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapour diffusion, hanging drop | 7.5 | 298 | 15% PEG 3350, 100mM KOAc, 1mM spermidine, pH 7.5, vapour diffusion, hanging drop, temperature 298K |
