3QRH
Crystal structure of fructose bisphosphate aldolase from Encephalitozoon Cuniculi, bound to glyceraldehyde 3-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.977400 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 122.540, 138.090, 62.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.460 - 2.000 |
R-factor | 0.164 |
Rwork | 0.163 |
R-free | 0.18700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mbf |
RMSD bond length | 0.016 |
RMSD bond angle | 1.395 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 | |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.052 | 0.019 | 0.438 |
Number of reflections | 36353 | 444 | 2650 |
<I/σ(I)> | 22.64 | 58.8 | 4.2 |
Completeness [%] | 99.8 | 94.3 | 100 |
Redundancy | 6 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 10% PEG6000, 0.1 M HEPES, protein at ~22 mg/ml, glyceraldehyde 3-phosphate at 10 mM., pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |