3QR7
Crystal structure of the C-terminal fragment of the bacteriophage P2 membrane-piercing protein gpV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2009-12-16 |
Detector | PSI PILATUS 6M |
Spacegroup name | H 3 2 |
Unit cell lengths | 49.069, 49.069, 463.896 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.327 - 0.940 |
R-factor | 0.1025 |
Rwork | 0.102 |
R-free | 0.11200 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.991 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX (C/D/E) |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.330 | 0.990 |
High resolution limit [Å] | 0.940 | 0.940 |
Rmerge | 0.063 | 0.282 |
Number of reflections | 141144 | |
<I/σ(I)> | 16.6 | 5.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 50 mg/mL protein, 30% PEE 797, 0.3 M ammonium sulfate, 0.1 M MES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |