3QR6
Crystal Structure Analysis of H185F Mutant of Human CLIC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2009-10-05 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.292, 82.081, 41.581 |
| Unit cell angles | 90.00, 95.84, 90.00 |
Refinement procedure
| Resolution | 41.040 - 1.780 |
| R-factor | 0.2046 |
| Rwork | 0.202 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k0m |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.968 |
| Data reduction software | SAINT |
| Data scaling software | SAINT (V7.34A) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.040 | 3.834 | 1.844 |
| High resolution limit [Å] | 1.780 | 3.044 | 1.780 |
| Number of reflections | 22460 | ||
| <I/σ(I)> | 271.079 | ||
| Completeness [%] | 97.7 | ||
| Redundancy | 4.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Bis-Tris, 30% (v/v) PEG 550 monomethyl ether, 0.05 M calcium chloride, 2.5 mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
