3QR5
Structure of the first domain of a cardiac Ryanodine Receptor mutant with exon 3 deleted
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-30 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9729 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 95.370, 44.204, 76.606 |
Unit cell angles | 90.00, 90.98, 90.00 |
Refinement procedure
Resolution | 38.290 - 2.300 |
R-factor | 0.2109 |
Rwork | 0.209 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.435 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.065 | 0.048 | 0.200 |
Number of reflections | 12917 | ||
<I/σ(I)> | 14 | ||
Completeness [%] | 84.5 | 98.8 | 32.9 |
Redundancy | 1.8 | 2 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10-15% PEG 1000. Crystals frozen in 25% 2-methane-2,4-pentanediol cryoprotectant, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |