3QQZ
Crystal structure of the C-terminal domain of the yjiK protein from Escherichia coli CFT073
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 130.024, 130.024, 130.024 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.550 |
| R-factor | 0.23179 |
| Rwork | 0.229 |
| R-free | 0.29067 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.636 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.550 |
| Rmerge | 0.084 |
| Number of reflections | 12816 |
| <I/σ(I)> | 37 |
| Completeness [%] | 100.0 |
| Redundancy | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M sodium Cacodylate, 0.2 M Calcium Acetate, 40% Peg 300, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
