3QQX
Reduced Native Intermediate of the Multicopper Oxidase CueO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.173, 91.375, 54.328 |
| Unit cell angles | 90.00, 102.22, 90.00 |
Refinement procedure
| Resolution | 24.350 - 1.500 |
| R-factor | 0.20774 |
| Rwork | 0.206 |
| R-free | 0.24226 |
| Starting model (for MR) | 1kv7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.540 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.070 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.055 | 0.338 |
| Number of reflections | 73797 | |
| <I/σ(I)> | 9.8 | 2.2 |
| Completeness [%] | 96.0 | 93 |
| Redundancy | 2.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 16% POLYETHYLENE GLYCOL 4000, 0.2 M AMMONIUM ACETATE, 0.1 M SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
