3QQT
Amphiphilic nanotubes in the crystal structure of a biosurfactant protein hydrophobin HFBII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 42.052, 91.359, 94.808 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.220 - 1.900 |
| R-factor | 0.2138 |
| Rwork | 0.211 |
| R-free | 0.26330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r2m |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.227 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 5.000 | 2.200 |
| High resolution limit [Å] | 1.900 | 4.000 | 1.900 |
| Rmerge | 0.089 | ||
| Number of reflections | 27376 | ||
| Completeness [%] | 98.4 | 99.9 | 96.4 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 15% polyethylene glycol MW 2000, 0.2 M lithium sulphate, 0.1 M Tris-HCl pH 8.5, Stock solution of polystyrene nanospheres, diameter 50 nm, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
