3QQ7
Crystal Structure of the p97 N-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9 |
Spacegroup name | P 31 |
Unit cell lengths | 60.560, 60.560, 63.110 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.280 - 2.650 |
R-factor | 0.1967 |
Rwork | 0.195 |
R-free | 0.23550 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.351 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 52.446 | 31.555 | 2.790 |
High resolution limit [Å] | 2.650 | 8.380 | 2.650 |
Rmerge | 0.050 | 0.021 | 0.573 |
Number of reflections | 7515 | ||
<I/σ(I)> | 16.4 | 23.3 | 1.3 |
Completeness [%] | 100.0 | 98.6 | 100 |
Redundancy | 4.5 | 4.4 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 1 M Hexanediol, 0.1 M Na-acetate, 0.01 M CoCl2, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |