3QQ5
Crystal structure of the [FeFe]-hydrogenase maturation protein HydF
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793, 0.9795, 0.9769 |
Spacegroup name | P 2 3 |
Unit cell lengths | 138.259, 138.259, 138.259 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.710 - 2.990 |
R-factor | 0.274 |
Rwork | 0.274 |
R-free | 0.30900 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.700 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.820 | 3.150 |
High resolution limit [Å] | 2.990 | 2.990 |
Rmerge | 0.120 | 0.484 |
Number of reflections | 18028 | |
<I/σ(I)> | 20.9 | 3.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 31.9 | 32.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.8 | 293 | 8% PGA-LM (Poly-L-glutamic acid, low molecular weight range), 0.2 M Na-Formate, 0.1 M Tris buffer, pH 7.8, VAPOR DIFFUSION, temperature 293K |
1 | VAPOR DIFFUSION | 7.8 | 293 | 8% PGA-LM (Poly-L-glutamic acid, low molecular weight range), 0.2 M Na-Formate, 0.1 M Tris buffer, pH 7.8, VAPOR DIFFUSION, temperature 293K |