3QPM
Crystal structure of peroxiredoxin Prx4 from Pseudosciaena crocea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-09 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.121, 195.998, 51.740 |
| Unit cell angles | 90.00, 105.23, 90.00 |
Refinement procedure
| Resolution | 37.980 - 1.900 |
| R-factor | 0.19682 |
| Rwork | 0.196 |
| R-free | 0.21976 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pn8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.281 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.066 | 0.214 |
| Number of reflections | 108135 | |
| <I/σ(I)> | 12.4 | 5.2 |
| Completeness [%] | 98.9 | 94.2 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 10% polyethylene glycol monomethyl ether 5000, 0.1M HEPES-NaOH, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
