3QOD
Crystal Structure of Heterocyst Differentiation Protein, HetR from Fischerella mv11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 65 |
| Unit cell lengths | 123.383, 123.383, 109.615 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.387 - 3.380 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.26700 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.896 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.410 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 13425 | |
| <I/σ(I)> | 9.3 | 3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.7 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 289 | 1.0 M ammonium sulphate, 0.1 M imidazole pH 8.0, 0.2 M sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
