3QO0
Monoclinic form of IgG1 Fab fragment (in complex with antigenic peptide) sharing same Fv as IgA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 108 |
Detector technology | IMAGE PLATE |
Collection date | 2009-12-04 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.320, 67.270, 68.880 |
Unit cell angles | 90.00, 103.10, 90.00 |
Refinement procedure
Resolution | 28.230 - 2.300 |
R-factor | 0.1712 |
Rwork | 0.169 |
R-free | 0.22140 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.280 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.9) |
Phasing software | FFT |
Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.232 | 28.232 | 2.420 |
High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
Rmerge | 0.023 | 0.572 | |
Rmeas | 0.027 | 0.672 | |
Rpim | 0.014 | 0.349 | |
Total number of observations | 2518 | 11220 | |
Number of reflections | 21241 | ||
<I/σ(I)> | 12.4 | 41.6 | 2.4 |
Completeness [%] | 99.9 | 98.3 | 100 |
Redundancy | 3.7 | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 293 | 0.1M Na citrate, 20% isopropanol, 16% PEG 4000, pH 6.1, vapor diffusion, sitting drop, temperature 293K |