3QNZ
Orthorhombic form of IgG1 Fab fragment (in complex with antigenic tubulin peptide) sharing same Fv as IgA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 108 |
Detector technology | IMAGE PLATE |
Collection date | 2009-12-14 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.370, 66.450, 137.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.210 - 2.200 |
R-factor | 0.1982 |
Rwork | 0.196 |
R-free | 0.23510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qo0 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.220 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.9) |
Phasing software | AMoRE |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 68.840 | 28.206 | 2.320 |
High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
Rmerge | 0.021 | 0.493 | |
Rmeas | 0.025 | 0.586 | |
Rpim | 0.014 | 0.312 | |
Total number of observations | 2840 | 12593 | |
Number of reflections | 25641 | ||
<I/σ(I)> | 14 | 42.2 | 2.6 |
Completeness [%] | 99.9 | 98.2 | 100 |
Redundancy | 3.4 | 3.2 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M Na citrate, 20% isopropanol, 16% PEG 4000, pH 5.5, vapor diffusion, sitting drop, temperature 293K |