3QNY
Monoclinic form of human IgA1 Fab fragment, sharing same Fv as IgG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 108 |
Detector technology | IMAGE PLATE |
Collection date | 2008-08-11 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 96.300, 50.000, 99.200 |
Unit cell angles | 90.00, 107.50, 90.00 |
Refinement procedure
Resolution | 18.750 - 2.300 |
R-factor | 0.1878 |
Rwork | 0.187 |
R-free | 0.21780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2EH7 (for the light chains) and 2FBJ (for the heavy chains) |
RMSD bond length | 0.010 |
RMSD bond angle | 1.240 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.500 | 27.500 | 2.400 |
High resolution limit [Å] | 2.300 | 10.000 | 2.300 |
Rmerge | 0.065 | 0.033 | 0.448 |
Rmeas | 0.039 | 0.527 | |
Number of reflections | 39517 | 500 | 4385 |
<I/σ(I)> | 13.44 | 30.8 | 3.16 |
Completeness [%] | 97.3 | 91.7 | 91.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 0.2M potassium sulfate, 20% PEG 3350, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |