3QNX
Orthorhombic form of human IgA1 Fab fragment, sharing same Fv as IgG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 108 |
Detector technology | IMAGE PLATE |
Collection date | 2009-06-18 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 49.780, 95.850, 107.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.150 - 2.200 |
R-factor | 0.1837 |
Rwork | 0.181 |
R-free | 0.22410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3m8o |
RMSD bond length | 0.010 |
RMSD bond angle | 1.220 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.9) |
Phasing software | AMoRE |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.925 | 47.925 | 2.320 |
High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
Rmerge | 0.040 | 0.490 | |
Rmeas | 0.047 | 0.580 | |
Rpim | 0.025 | 0.305 | |
Total number of observations | 3087 | 13435 | |
Number of reflections | 26858 | ||
<I/σ(I)> | 9.8 | 22.8 | 2.6 |
Completeness [%] | 100.0 | 99.3 | 100 |
Redundancy | 3.5 | 3.2 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.8 | 293 | 1.8M (NH4)2SO4, 0.1M PO4/citrate, pH 3.8, vapor diffusion, sitting drop, temperature 293K |