3QNU
Crystal structure of the cytosolic domain of human atlastin-1 in complex with GDP, hexagonal form
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-14 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9793, 0.9795 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 144.889, 144.889, 103.694 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.966 - 2.800 |
R-factor | 0.2385 |
Rwork | 0.235 |
R-free | 0.30610 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.466 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 50.000 | 3.020 |
High resolution limit [Å] | 2.800 | 4.790 | 2.800 |
Rmerge | 0.071 | 0.040 | 0.330 |
Number of reflections | 16292 | ||
<I/σ(I)> | 14.5 | 122 | 6.4 |
Completeness [%] | 99.9 | 100 | 99.9 |
Redundancy | 13.6 | 15 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris-HCl, pH8.5, 25%(w/v) PEG1500, 20%(v/v) Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |