3QMX

X-ray crystal structure of Synechocystis sp. PCC 6803 Glutaredoxin A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	90
Detector technology	CCD
Collection date	2010-06-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	37.278, 39.124, 50.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.060 - 1.822
R-factor	0.1664
Rwork	0.163
R-free	0.19560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3grx
RMSD bond length	0.005
RMSD bond angle	0.952
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.060
High resolution limit [Å]	1.822
Rmerge	0.077
Number of reflections	6614
Completeness [%]	94.1
Redundancy	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	1% PEG 4000, 0.1M HEPES, 1.25M Ammonium Sulfate, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K