3QMA
Blackfin tuna myoglobin imidazole complex, atomic resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.198, 52.381, 39.022 |
| Unit cell angles | 90.00, 104.43, 90.00 |
Refinement procedure
| Resolution | 16.780 - 0.940 |
| R-factor | 0.1471 |
| Rwork | 0.146 |
| R-free | 0.16039 |
| Structure solution method | RIGID BODY refinement |
| Starting model (for MR) | 2nrl |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.602 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.780 | 0.965 |
| High resolution limit [Å] | 0.940 | 0.940 |
| Rmerge | 0.065 | 0.469 |
| Number of reflections | 79945 | |
| <I/σ(I)> | 27.4 | 2.32 |
| Completeness [%] | 97.5 | 88.35 |
| Redundancy | 5.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M potassium/sodium tartrate, 0.1 M sodium citrate, 2.0 M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
