3QM7
Blackfin tuna carbonmonoxy-myoglobin, atomic resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-06 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.92 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.286, 51.714, 38.633 |
Unit cell angles | 90.00, 105.09, 90.00 |
Refinement procedure
Resolution | 17.360 - 0.960 |
R-factor | 0.15279 |
Rwork | 0.152 |
R-free | 0.17182 |
Structure solution method | RIGID BODY refinement |
Starting model (for MR) | 2nrl |
RMSD bond length | 0.014 |
RMSD bond angle | 1.581 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.360 | 0.986 |
High resolution limit [Å] | 0.960 | 0.960 |
Rmerge | 0.060 | 0.504 |
Number of reflections | 73608 | |
<I/σ(I)> | 28.04 | 2.2 |
Completeness [%] | 97.9 | 89.24 |
Redundancy | 5.4 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M potassium/sodium tartrate, 0.1 M sodium citrate, 2.0 M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |