3QLT
Crystal structure of a GluK2 (GluR6) glycan wedge homodimer assembly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-24 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | I 4 |
Unit cell lengths | 191.805, 191.805, 47.009 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.951 - 2.988 |
R-factor | 0.2441 |
Rwork | 0.242 |
R-free | 0.29320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h6g |
RMSD bond length | 0.003 |
RMSD bond angle | 0.608 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.100 |
High resolution limit [Å] | 2.988 | 2.990 |
Rmerge | 0.078 | 0.698 |
Number of reflections | 17783 | |
<I/σ(I)> | 16 | 2 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10% PEG 8K, 20% ethylene glycol, 0.02 M each 1,6-hexanediol, 1-butanol, (RS)-1,2-propanediol, 2-propanol, 1,4 butanediol, 1,3-propanediol, and 0.1 M MOPS/HEPES-Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |