3QLL
Crystal Structure of RipC from Yersinia pestis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9787 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 88.056, 88.056, 197.021 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.970 - 2.450 |
R-factor | 0.232 |
Rwork | 0.230 |
R-free | 0.25600 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.199 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX (dev_886) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
High resolution limit [Å] | 2.450 | 7.590 | 2.450 |
Rmerge | 0.061 | 0.013 | 0.382 |
Number of reflections | 43062 | ||
<I/σ(I)> | 13.1 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 5.8 | 5.9 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 298 | 18% ETHYLENE GLYCOL, .1M TCEP HYDROCHLORIDE, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |