3QLK
Crystal Structure of RipA from Yersinia pestis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2009-11-14 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 197.508, 197.508, 61.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.716 - 3.000 |
R-factor | 0.1864 |
Rwork | 0.184 |
R-free | 0.21680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.627 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.120 | 0.040 | 0.563 |
Number of reflections | 27624 | ||
<I/σ(I)> | 7.2 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 14.6 | 14.6 | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 298 | 18% PEG 3350, .2M Na Citrate, .1M BisTris Propane pH 6.3 and 1mM CoA, VAPOR DIFFUSION, HANGING DROP, temperature 298K |