3QLE

Structural Basis for the Function of Tim50 in the Mitochondrial Presequence Translocase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2008-10-19
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9792
Spacegroup name	P 61 2 2
Unit cell lengths	84.109, 84.109, 116.549
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.989 - 1.831
R-factor	0.1762
Rwork	0.175
R-free	0.20160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ghq
RMSD bond length	0.008
RMSD bond angle	1.186
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_351))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.830
Number of reflections	21756
Completeness [%]	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	290	100 mM MES buffer (pH 6.0), 30% (w/v) PEG4K and 0.2 M ammonium acetate, EVAPORATION, temperature 290K