3QKU
Mre11 Rad50 binding domain in complex with Rad50 and AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-01 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 177.398, 177.398, 130.423 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 3.300 |
R-factor | 0.26052 |
Rwork | 0.258 |
R-free | 0.30838 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1us8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.223 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 153.600 | 3.387 |
High resolution limit [Å] | 3.300 | 3.301 |
Rmerge | 0.048 | 0.660 |
Number of reflections | 18667 | |
<I/σ(I)> | 35.7 | 3.6 |
Completeness [%] | 99.7 | 99.41 |
Redundancy | 9.8 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris, 200-300 mM lithium sulfate, 12-13% PEG3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |