3QKT
Rad50 ABC-ATPase with adjacent coiled-coil region in complex with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.390, 108.517, 150.366 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.651 - 1.900 |
| R-factor | 0.1947 |
| Rwork | 0.192 |
| R-free | 0.24360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1us8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.970 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.995 | 1.922 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.056 | 0.480 |
| Number of reflections | 105235 | |
| <I/σ(I)> | 25.4 | 1.9 |
| Completeness [%] | 97.5 | 81.8 |
| Redundancy | 5.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 100 mM Tris, 100 mM sodium chloride, 16-18% PEG550 MME, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
