3QKT
Rad50 ABC-ATPase with adjacent coiled-coil region in complex with AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-01 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.390, 108.517, 150.366 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.651 - 1.900 |
R-factor | 0.1947 |
Rwork | 0.192 |
R-free | 0.24360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1us8 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.970 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.995 | 1.922 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.056 | 0.480 |
Number of reflections | 105235 | |
<I/σ(I)> | 25.4 | 1.9 |
Completeness [%] | 97.5 | 81.8 |
Redundancy | 5.3 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 100 mM Tris, 100 mM sodium chloride, 16-18% PEG550 MME, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |