3QKG
Crystal structure of alpha-1-microglobulin at 2.3 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2011-04-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9184 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 66.721, 66.721, 80.255 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.890 - 2.300 |
R-factor | 0.21478 |
Rwork | 0.213 |
R-free | 0.25952 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.703 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MrBUMP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.890 | 2.290 |
High resolution limit [Å] | 2.170 | 2.170 |
Rmerge | 0.073 | 1.380 |
Number of reflections | 10877 | |
Completeness [%] | 96.5 | 80 |
Redundancy | 10.4 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100 mM Tris-HCl, 1.1 M sodium citrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |