3QKC
CRYSTAL STRUCTURE OF geranyl diphosphate synthase small subunit from Antirrhinum majus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.199, 48.789, 61.593 |
| Unit cell angles | 66.69, 89.28, 89.98 |
Refinement procedure
| Resolution | 19.930 - 2.200 |
| R-factor | 0.2132 |
| Rwork | 0.209 |
| R-free | 0.28880 |
| Structure solution method | SAD, MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.321 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 5.560 | 2.050 |
| Rmerge | 0.073 | 0.060 | 0.698 |
| Number of reflections | 28005 | ||
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 97.2 | 96.4 | 87.7 |
| Redundancy | 2.9 | 2.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 M Hepes, 1.4 M sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
