3QKC

CRYSTAL STRUCTURE OF geranyl diphosphate synthase small subunit from Antirrhinum majus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 1
Unit cell lengths	43.199, 48.789, 61.593
Unit cell angles	66.69, 89.28, 89.98

Refinement procedure

Resolution	19.930 - 2.200
R-factor	0.2132
Rwork	0.209
R-free	0.28880
Structure solution method	SAD, MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.321
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.090
High resolution limit [Å]	2.050	5.560	2.050
Rmerge	0.073	0.060	0.698
Number of reflections	28005
<I/σ(I)>	11.6
Completeness [%]	97.2	96.4	87.7
Redundancy	2.9	2.9	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M Hepes, 1.4 M sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K