3QK3
Crystal structure of human beta-crystallin B3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.778, 82.627, 63.592 |
| Unit cell angles | 90.00, 97.92, 90.00 |
Refinement procedure
| Resolution | 19.630 - 1.950 |
| R-factor | 0.1734 |
| Rwork | 0.172 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oki |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.630 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.066 | 0.429 |
| Number of reflections | 35273 | |
| <I/σ(I)> | 10.5 | 2.1 |
| Completeness [%] | 95.7 | 94.9 |
| Redundancy | 2.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES, 1.5M Li2SO4, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
