3QJY
Crystal structure of P-loop G234A mutant of subunit A of the A1AO ATP synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.182, 128.182, 105.788 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.830 - 2.350 |
| R-factor | 0.23445 |
| Rwork | 0.233 |
| R-free | 0.26315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vdz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.095 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 5.060 | 2.350 |
| Rmerge | 0.055 | 0.023 | 0.437 |
| Number of reflections | 37307 | ||
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 99.9 | 99.2 | 100 |
| Redundancy | 7.2 | 6.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 50% (v/v) MPD, 2mM MgADP, 0.1M acetate (pH 4.5), vapor diffusion, hanging drop, temperature 291K |
